Part:BBa_K4417015

CuO-RBS-ureABC-rrnB T1 Terminator TU1B

Description

This part is the coding sequence of ureEFG accessory proteins from Sporosarcina pasteurii. It was cloned into the master plasmid (BBa_K4417017).

This part is not Type IIS compatible as it has BsaI sites.

Figure 1: Basic part ureEFG TU2B

Usage and Biology

• This basic part can be used to produce urease accessory proteins.
• It can be cloned into the master plasmid (BBa_K4417017) to express the nature urease operon from Sporosarcina pasteurii using Golden Gate assembly.
• The part was synthesized by IDT as a gBlock.

Reference

1. Zerner, B. “Recent advances in the chemistry of an old enzyme, urease.” Bioorg. Chem. 19 (1991):116-131

2. Krajewska, Barbara. "Ureases I. Functional, catalytic and kinetic properties: A review". Journal of Molecular Catalysis B: Enzymatic 59 no.1-3 (2009):9–21. doi:10.1016/j.molcatb.2009.01.003

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 969
  Illegal BamHI site found at 1
  Illegal XhoI site found at 1723
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 7
  Illegal BsaI.rc site found at 2066